Abstract: By using the data of land-use types from remote sensing images of Anhui Province in 2000, 2005 and 2010 with ArcGIS, the ecosystem service values of different land types of Anhui Province are calculated from the service intensity and location index on the basis of LUCC; furthermore, the spatial and temporal changes are analyzed. The results show that on the distribution of land types, the northern plain area (Suzhou, Huaibei, etc) is single arable land; the southern (Huangshan, etc) and western area (Lu'an, etc) is a combined type, a gradual transformation from single to diversity is manifested from north to south; from 2000 to 2010, the service value of ecosystem of Anhui Province had decreased by 0.7%, the service intensity of farmland+ wetland+ forests accounted for 94.76% of the whole province, and its service value of ecosystem occupied 92.98% of whole province. Among the cities of Anhui Province, the larger the portion of farm land, wetland and forests is, the greater the service value of the ecosystem is. The land-use change is a major factor to the service value variation of ecosystem of Anhui Province.