Abstract: The water supply from Songhuajiang River of Jilin Province is one of the 172 major water conservancy projects in China recently, and it is also a major trans-region water diversion project that is characterized as the largest investment, the longest line, the largest benefited area and most difficult construction difficulty in history of Jinlin Province. It is one of the key technical problems in the project that whether the prestressed culvert with inner diameter of 5.1m can be successfully applied. Through the 1:1 prototype structure model test, the key scientific problems such as the changing law of prestress of steel cable, the friction resistance along the channel and the loss of friction resistance of tensioned anchor head, the stress and deformation law of concrete culvert under different working conditions, and the deformation law of expansion joint are systematically studied. Through a series of tests, we made achievements in the following test technologies, ground hydraulic pressure test seal technology for prestressed culverts, the distributed optical fiber sensor technology, magnetic effect testing technology, underwater convergence deformation testing technology, the double helix prestressed culvert design method, gas-liquid composite pressure regulator technology, the three-dimensional nonlinear numerical simulation technology of culvert prestressed structure and model test technology under extreme cold weather. Based on these results, we successfully optimized the design scheme, and improved construction technology. The research results provide technical support for the development of design method of prestressed water transmission culvert in water conservancy and hydropower projects.