Abstract: Seismic isolation technology, a useful and important measure for improving anti-seismic capacity of bridges, has been successfully applied in many countries to protect buildings and bridges from damage caused by strong earthquakes．In order to make the elevated aqueduct structure in high intensity area have good seismic isolation performance and automatic reset function after earthquakes, a bevel guide automatic reset and seismic isolation bearing is developed based on spherical bearing. The structural characteristics and isolation mechanism of the automatic reset are introduced first. Then a horizontal resilience test and a vibration test of single-trough aqueduct with this bearing are carried out. The results show that the new type bearing has stable seismic isolation performance and good automatic reset effect, as it could effectively reduce horizontal force at the column top and thus reduce bending moment at column base while has small residual deformation.