Abstract: Based on the data of the second national pollution source census in China, the spatial distribution characteristics of industrial water pollution point sources in Yunnan Province are explored from different angles such as industry, water system and administrative area by using the methods of standard deviation ellipse analysis and nuclear density analysis.The results show that:(1) the industries with the largest number of point sources are mainly wine, beverage and refined tea manufacturing(C-15),agricultural and sideline food processing(C-13),food manufacturing(C-14),water production and supply(D-46),etc.The top three emission industries of chemical oxygen demand(COD) are C-13,C-14 and C-15,and the top three emission industries of ammonia nitrogen are C-13,C-14 and C-26(chemical raw materials and chemical products manufacturing).(2) COD emissions are mainly located in Yuxi City, Dehong Prefecture, Lijiang City and Qujing City, accounting for 90.77% of the province; ammonia nitrogen emissions are concentrated in Dali Prefecture, Honghe Prefecture, Yuxi City and Chuxiong Prefecture, accounting for 81.93%.(3) The main industries of point source emissions have directionality in spatial distribution and form a relatively concentrated trend.The aggregation centers are located in Central Yunnan Province, and most of the aggregation districts and counties with high pollution concentration are located in or adjacent to the state and municipal government location.The point sources and concentration values of water pollutants are generally consistent in spatial distribution, but different in a few areas.The research results can provide theoretical basis for formulating water pollution control measures in Yunnan Province.