Abstract: The substrate of constructed wetland system plays an important role in the removal of pollutants in sewage, but there is a lack of research on the co-treatment of eutrophic substances and emerging pollutants by constructed wetlands.In this paper, three different types of substrates, gravel, biochar and zeolite, were selected to construct a vertical subsurface flow constructed wetland to evaluate the removal efficiency of constructed wetland on ciprofloxacin and nitrogen and phosphorus in sewage.The results showed that when the wetland substrate was replaced with biochar and zeolite from gravel, the removal efficiency of ciprofloxacin increased from 69.6% to 79.9% and 88.6%.Simultaneously, the removal efficiency of total nitrogen increased from 47.8% to 49.1% and 76.1%,and total phosphorus increased from 37.7% to 46.3% and 48.9%.Compared with gravel and biochar substrates, zeolite-based constructed wetland exhibited a better removal efficiency on both ciprofloxacin and eutrophic substances.