Abstract: In order to deeply explore the flow-induced vibration characteristics on the deep-hole radial working gate of the Three Gorges Dam during operation, prototype observation study was carried out.The time-history analysis and spectrum analysis results showed that when the gate was opened and closed dynamically, the extreme values of vibration dynamic displacement and acceleration were relatively small, and the vibration was small.At the moment of opening and stopping, the dynamic displacement extreme value and acceleration extreme value of the gate increased obviously, and the vibration was large.When the gate opening was 0.6～0.7,the forced vibration of the gate occurred.The No.1 and No.12 deep hole radial gates were affected by flow-induced vibration to varying degrees during operation, which was small or medium vibration in the safe range.The relevant results can be used as reference by dam safety operation personnels.