Abstract: To study the response relationship of different land use types to carbon sequestration effects after ecological restoration, based on spatial geographic information such as land use data and vegetation cover type data in Qingzhen City Guizhou Province in2000and2020,the vegetation carbon storage in sloping land, grassland, shrub land and arbor land were estimated at a wide spatial scale.The content and storage of soil organic carbon components, Karst rate, and soil CO2concentration were measured.Then the carbon sequestration effect of the ecosystem was compared and assessed from the perspective of vegetation carbon storage, soil organic carbon content, and the Karst effect.The results showed that:(1) In2022,the total carbon storage of vegetation of four land-use types amounted to19.42×105t, showing a1.83×105t increase from2000.Arbor land comprised95.73% of the total vegetation carbon storage.As for the carbon density, the carbon density among different types of vegetation from high to low was: evergreen coniferous forest > evergreen broad-leaved forest > deciduous broad-leaved forest> shrub forest> crop> grassland.(2)Compared to sloping land, grassland, shrub land, and arbor land exhibited notable increases in SOC,POC,MOC,and EOC.The soil organic carbon component storage of the four land use types from high to low were arbor land > shrub land > grass land > sloping land.(3) The dissolution rate was directly correlated with the concentration of soil CO2.Both soil dissolution and soil organic carbon content increased with the increase in CO2concentration.