Abstract: The comprehensive evaluation on ecological cleaning degree of Jianliupu small watershed has important guiding effect for the subsequent construction of Jianliupu small watershed. In 2016, the comprehensive evaluation on ecological cleaning degree of Jianliupu small watershed was carried out through wild surveys and monitoring, 3S technology and laboratory analysis respectively. The research results demonstrate that the ecological cleaning degree of Jianliupu small watershed is relatively clean. Trench aquatic plant diversity, the water environmental quality of the small watershed estuary, soil and water conservation rate and the domestic sewage treatment rate of village and towns are the main reasons influencing ecological cleaning degree of Jianliupu small watershed. Replacing the comprehensive governance rate about the loss of water and single-factor evaluation method in the original evaluation by the soil and water conservation rate and the F-value assessment method can improve the scientificity and comprehensiveness. Research results can provide a reference for comprehensive evaluation on ecological cleaning degree in small watersheds in Hubei Province and even nationwide.